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Lateral Flow Membrane | Nitrocellulose Membrane | Axiva

Nitro Cellulose Lateral Flow Membrane

Introduction

Established in year 2006, Axiva Sichem Pvt. Ltd. is engaged in manufacturing & exporting a wide range of Diagnostic products like
1. Nitrocellulose Lateral Flow Membrane
2. Sample Pad (Glass Fibre)
3. Conjugate Pad (Polyester Pad)
4. Absorbent Pad (Cellulose Fibre)
5. Membrane Filters

Please Refer to Our Latest Website for IVD Products :- https://axiflow.co.in/product/lateral-flow-membrane

Nitrocellulose Lateral Flow Membrane

Nitrocellulose Membrane is the most important part of a lateral flow assay and require highly consistent measurements for capillary flow rate and thickness across the length and width. Variation in either can have detrimental effects on assay sensitivity, reproducibility, repeatability in results and reagent consumption (in special assay developments which are controlled by time or volume consumed). Signal generation at the test and control lines is influenced by the thickness of the reaction membrane. The variation in thickness across or down a roll can have negative effects in both the manufacturing in-process approval levels and final assay performance.

For maintaining the uniformity of membrane Axiva posses a complete in house developed manufacturing unit for membrane and well developed measuring techniques which ensure release of product with stringent specification and CV for the best performance under critical assay developing processes.

cellulose-leateral-flow-membrane

Consistency By Design

The Team
A group of skilled scientists, technicians, production workers and management are contributing their ability in a team work for developing a membrane technology for Lateral Flow Assay Test.

The Application Lab
Our diagnostic membrane application lab is fully equipped with instruments and material that enable us to characterize diagnostic membrane. To evaluate for Lateral Flow Test almost common chemistries and parameters are used in production and in laboratory.

Manufacturing Facilities
Axiva using a complete clean room in-house production facility well equipped with instruments. We have rigid environmental controls in place to produce a stable and clean manufacturing process. Complete area is class 100000, GMP compliant.

Membrane Manufacturing Process
The solution mixing and casting processes are designed and controlled to provide outstanding inter and intra-lot consistency. AXIVA owns clean-room facility for production of membrane which ensures a dust free smooth membrane surface.

Consistency By Testing

Physical Testing
Capillary wicking timeis simple and highly informative test. 50μl of normal saline solution dropped at one edge of membrane strip and it wicks towards an open edge. Time taken to reach a fixed distance is called wicking time in seconds. Axiva membranes are characterised and differentiated by measurements of Wicking times of well planned samples and pore size comparisons with microscope. So we have traceable, repeatable and consistent quality products with well defined wicking times in seconds with isotonic normal saline solution. Signal generation at the time of test, control lines and tensile strength are largely influenced by the thickness of the reaction membrane. The variation in thickness across or down a roll can have negative effects in both the manufacturing environment and final assay performance. For maintaining the uniformity of membrane Axiva posses a complete in-house manufacturing for membrane and measurements are highly controlled with specification and CV for the critical parameters of membrane.

Functional Properties
Protein binding is a critical aspect of Nitrocellulose Membrane performance. To achieve optimum assay results, the membrane must bind sufficient proteins at the capture lines but not non-specifically bind the target analytes in the sample. Assay reproducibility is facilitated by consistent levels of protein binding which translates to achieving the desired amount of antibody binding at the capture lines. If there is variability in protein binding at the capture lines, the assay sensitivity and crispness of the capture line can be compromised. Axiva produces a uniform and good binding membrane that better for assay testing.

Consistent Wettability
Although cellulose nitrate polymers are naturally hydrophobic, Axiva use specific surfactant for treatment, to get a long time uniform wetting property of membranes. Also stability studies are done over a period of time on the basis of accelerated heat stability test Data of storage at normal storage conditions

Good Quality’s that increase the assay reproducibility and sensitivity of membrane

Strong Consistency – Membrane provides high sensitivity and low background in diagnostic assays due to its consistent nature.

Increased sensitivity – Provides high sensitivity with dilute concentrations of target analytes with no false signals

Clear results – Demonstrates low background levels which enable crisp capture lines and easy-to-read results.

Dependable performance – Constructed of pure nitrocellulose that does not contain interfering substances or post-treatments that affect assay performance.

How to select nitrocellulose lateral flow membrane?
Selection of LFT membrane depends upon assay.Highest sensitivity is obtained with smaller size and slower wicking time/capillary flow time.Axiva offer wide range of Nitrocellulose lateral flow membrane to meet the requirement of different tests.

Wicking time/Capillary flow timeMembrane Micron ratingSensitivityFlow RateLine intensityApplications
160 ± 25
sec,40 mm
distance
8 µmHighestSlowerSharpMilk test, Urine Ab Test, HbsAg serum test
130 ± 15
sec/40 mm
distance
10 µmMediumMediumSharpDengue, Malaria Infectious disease screening, Environmental and agricultural analytes.
115 ± 15
sec/40 mm
distance
12 µmMediumFast MediumSharpHeart Disease markers, Drug of abuse monitoring tests, Syphilis, Food and beverage testing
100 ± 15
sec/40 mm
distance
15 µmLowerFasterLess SharpWhole Blood tests, Monitoring ovulation, Dengue and infectious disease.
95 ± 15
sec/40 mm
distance
15 µmLowerFasterLess SharpPregnancy test.
90 ± 10
sec/40 mm
distance
90 secLowerFasterLess SharpPregnancy test.

1. Axiva Nitrocellulose Membrane 160 sec (8μm) Membrane Code DE
This membrane is used for testing of urine Ab tests and for HbsAg serum test.
Specification and characteristics

Backing SpecificationMembrane Specification
Material - PolyesterMaterial - Cellulose Nitrate Polymer
Thickness - 102 ± 5µmThickness - 105 ± 15µm (Membrane layer only)
Colour - TransparentWicking Time - 160 ± 25 sec/40mm distance
Wetting Time ≤ 5 sec (µl volume 0.3% BAS in PBS 0.01M pH 7.4)
Roll Specification
Roll Core - 76.5 mm = 3" (inner Diameter)
Core Material - Plastic
Available Membrane Width - As Desired

ORDERING INFORMATION :

Cat. No.RatingWicking TimeWidthLength
DE20/SX048µm160 Sec20 mm100 meter roll
DE25/SX048µm160 Sec25 mm100 meter roll
DE30/SX048µm160 Sec30 mm100 meter roll
DE40/SX048µm160 Sec40 mm100 meter roll

2. Axiva Nitrocellulose Membrane 130 sec (10μm) Membrane Code DF
This membrane is used for testing of Dengue, Malaria and for infection disease tests with serum etc.
Specification and characteristics

Backing SpecificationMembrane Specification
Material - PolyesterMaterial - Cellulose Nitrate Polymer
Thickness - 102 ± 5µmThickness - 105 ± 15µm (Membrane layer only)
Colour - TransparentWicking Time - 130 ± 15 sec/40mm distance
Wetting Time - ≤ sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4)
Roll Specification
Roll Core - 76.5 mm = 3" (inner Diameter)
Core Material - Plastic
Available Membrane width - As desired

ORDERING INFORMATION :

Cat. No.RatingWicking TimeWidthLength
DF20/SX0310µm130 Sec20 mm100 meter roll
DF25/SX0310µm130 Sec25 mm100 meter roll
DF25/SX0310µm130 Sec30 mm100 meter roll
DF25/SX0310µm130 Sec40 mm100 meter roll

*Custom dimensions are also available on request

3. Axiva Nitrocellulose Membrane 115 sec (12μm) Membrane Code CG
This membrane is used for testing of hCG, and for infection disease tests with serum etc.
Specification and characteristics

Backing SpecificationMembrane Specification
Material - PolyesterMaterial - Cellulose Nitrate Polymer
Thickness - 102 ± 5µmThickness - 105 ± 15µm (Membrane layer only)
Colour - TransparentWicking Time - 100 ± 15 sec/40mm distance
Wetting Time - ≤ 5 sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4)

ORDERING INFORMATION :

Cat. No.RatingWicking TimeWidthLength
CG20/SX0212µm115 Sec20 mm100 meter roll
CG25/SX0212µm115 Sec25 mm100 meter roll
CG30/SX0212µm115 Sec30 mm100 meter roll
CG40/SX0212µm115 Sec40 mm100 meter roll

*Custom dimensions are also available on request

4. Axiva Nitrocellulose Membrane 100 sec (15μm) Membrane Code CH
This membrane is used for whole blood test and for malaria tests. Due to larger pore size the wicking speed is fast and good for viscous or particle loaded sample.
Specification and characteristics

Backing SpecificationMembrane Specification
Material - PolyesterMaterial - Cellulose Nitrate Polymer
Thickness - 102 ± 5µmThickness - 105 ± 15µm (Membrane layer only)
Colour - TransparentWicking Time - 100 ± 15 sec/40mm distance
Wetting Time - ≤ 5 sec (10µl volume 0.3% BAS in PBS 0.01M pH 7.4)

ORDERING INFORMATION :

Cat. No.    
CH20/SX0515µm100 sec20 mm100 meter roll
CH20/SX0515µm100 sec25 mm100 meter roll
CH20/SX0515µm100 sec30 mm100 meter roll
CH20/SX0515µm100 sec40 mm100 meter roll

*Custom dimensions are also available on request

5. Axiva Nitrocellulose Membrane 90 sec Membrane Code BQ
This membrane is used for testing of hCG, for whole blood test and for Malaria tests. Due to larger pore size the wicking speed is fast and good for viscous or particle loaded sample.
Specification and characteristics

Backing SpecificationMembrane Specification
Material - PolyesterMaterial - Cellulose Nitrate Polymer
Thickness - 102 ± 5µmThickness - 105 ± 15µm (Membrane layer only)
Colour - TransparentWicking Time - 90 ± 10 sec/40mm distance
Wetting Time - 5 sec ≤ (10µm volume 0.3% BAS in PBS 0.01M pH 7.4)

ORDERING INFORMATION :

Cat. No.Wicking TimeWidthLength
BQ20/SB0190 sec20 mm100 meter roll
BQ25/SB0190 sec25 mm100 meter roll
BQ30/SB0190 sec30 mm100 meter roll
BQ40/SB0190 sec40 mm100 meter roll

*Custom dimensions are also available on request

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