BLOTTING MEMBRANE

A blot, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA, on to a carrier (for e.g. a nitrocellulose or nylon membrane).

SOUTHERN BLOT

This is a method routinely used in molecular biology for detection of specific DNA sequence in the sample

Northern Blot

This is a method routinely used in molecular biology for detection of specific DNA sequence in the sample

Western Blot

This is a method routinely used in molecular biology for detection of specific DNA sequence in the sample

BLOTTING MEMBRANE SELECTION CHART

HR = Highly Recommended R = Recommended

P = Possible NR = Not Recommended

Membrane Type	Nitrocellulose	Nylon
Nucleic Acid	R	HR
Proteins	HR	R
Transfer Method
Dot Blot	R	R
Clony / Plaque lift	HR	R
Electrotransfer	R	HR
Capillary Blot	R	R
Vaccum Blot	R	R
Alkaline Transfer	NR	R
Molecule Fixation Baking	R	R
Molecule Fixation Baking	R	R
Drying	R	R
UV Crosslinking	P	HR
Alkali Fixation	NR	R
Molecule Removal	NR	NR
Detection Method Colorimetric	HR	R
Radiolabeled	R	R
Luminesence	R	P
Fluorescence	R	P
Staining	R	P
Reprobing
Once	NR	R
Multiple	NR	R

NITROCELLULOSE TRANSFER (BLOTTING) MEMBRANE

ORDERING INFORMATION :

Cat No.	Size	Micron Rating	Nos./Pkt.
160200 XI	200 mm X 200 mm	0.45	10
160200 XI	200 mm X 200 mm	0.45	10
160100 XI	100 mm X 100 mm	0.45	10
S045A330 R	300 mm X 3 mts	0.45	One Roll
160300 RI	300 mm X 300 mm	0.2	10
160200 RI	200 mm X 200 mm	0.2	10
160100 RI	100 mm X 100 mm	0.2	10
S020A330 RI	300 mm X 3 mts	0.2	One Roll

AXIVA – NYLON TRANSFER (BLOTTING) MEMBRANE

ORDERING INFORMATION :

Cat. No.	Size	Micron Rating	Nos./Pkt.
170300 XI	300 mm X 300 mm	0.45	10
170200 XI	200 mm X 200 mm	0.45	10
170100 XI	100 mm X 100 mm	0.45	10
170330 XI	300 mm X 3 mts	0.45	One Roll
170300 RI	300 mm X 300 mm	0.2	10
170200 RI	200 mm X 200 mm	0.2	10
170100 RI	100 mm X 100 mm	0.2	10
170330 RI	300 mm X 3 mts	0.2	One Roll

AXIVA – CHROMATOGRAPHY PAPERS-R SERIES
Grade -1 CHR
ORDERING INFORMATION :

Cat. No.	Size Cm	Nos/Pkt.	Nos/Pkt. Std. box
111465R	46 X 57 cm	100	5 PKT.
111046R	46 X 57 cm	25	5 PKT.
111040R	40 X 50 cm	25	5 PKT.

AXIVA – THICK CHROMATOGRAPHY PAPERS-R
Grade -3 CHR
ORDERING INFORMATION :

Cat. No.	Size Cm	Nos/Pkt.	Nos/Pkt. Std. box
113465R	46 X 57 cm	50	1 PKT.
113657R	46 X 57 cm	100	1 PKT.

AXIVA – PURE CELLULOSE BLOTING SHEET SERIES
Grade -BF-3
ORDERING INFORMATION :

Cat. No.	Size Cm	Nos/Pkt.
2.520.5860 K	58 X 60 cm	50
2.520.4657 K<	46 X 57 cm	50
2.250.1010 K	10 X 10 cm	50
2.250.1515 K	15 X 15 cm	50
2.520.2020 K	20 X 20 cm	50

Blotting Paper

BLOTTING MEMBRANE